Journal: iScience
Article Title: Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila
doi: 10.1016/j.isci.2026.115209
Figure Lengend Snippet: ePLs alter membrane properties of Drosophila cells (A) Measurement of membrane tension using AFM in S2R+ cells (control) and cells supplemented with 100 μM 18-AG. Data were acquired from 242 different regions of 24 control cells and 213 different regions of 27 cells supplemented with 18-AG. The red horizontal lines represent the median, and each point is the average Young’s modulus (Pa) of each region. (B) Measurement of membrane tension with fluorescence lifetime imaging using Flipper-TR in control ( n = 45) and 100 μM 18-AG supplemented S2R+ cells ( n = 40). Red horizontal lines represent the median and each point indicates the average fluorescent lifetime of a single cell. (C) Representative pseudocolor images of LipiORDER loaded control (upper) and 100 μM 18-AG supplemented S2R+ cells (lower). Green fluorescence intensity (left, 450–550 nm), red fluorescence intensity (middle, 550–650 nm), and ratiometric images of red to green fluorescence intensity (right, R/G ratio). Scale bars, 100 μm. (D) Quantification of red/green fluorescence ratio (R/G ratio) in control ( n = 46) and 100 μM 18-AG supplemented S2R+ cells ( n = 45). Each value indicates the average R/G ratio of a single cell. Red horizontal lines represent the median, and each point is a biological replicate. Data are presented as mean ± SEM. ∗ p < 0.05 and ∗∗∗ p < 0.001; Mann-Whitney U test. (E) The calculated Laurdan GP score. Each value indicates the average GP score of a single cell. Red horizontal lines represent the median, and each point is a biological replicate. ∗ p < 0.05; Mann–Whitney U test. See also .
Article Snippet: Cells were plated on a non-coat glass bottom dish (35 mmφ, No.1S, Matsunami) and incubated for 24 h. Subsequently, the cells on the glass were washed with the same bath solution used for electrophysiological experiments and incubated with either 2 μM Flipper-TR (Spirochrome) or 100 nM LipiORDER (Funakoshi) in the bath solution for 15 min at room temperature.
Techniques: Membrane, Control, Fluorescence, Imaging, Single Cell, MANN-WHITNEY
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